T&L Biotechnology

Product Name

Generic Name

Recombinant Human IL-7 Protein

Synonym

IL7,Interleukin-7

Product Information

Construction

A DNA sequence encoding the human IL-7(P13232-1:D26-H177)was expressed with a Fc-tag at the C-terminus.

Expression Host

CHO cells

QC Testing Purity

> 95 % as determined by SDS-PAGE and HPLC

Activity

Cell proliferation assay was performed on PBMC cells activated with CD3 monoclonal antibody, with an ED50 of 2-20ng/mL and a corresponding specific activity of >1× 109Units/mg (calibrated according to human IL-7 reference standard (NIBSC code: 90/530))

Endotoxin

< 0.01EU per 1μg of the protein by the LAL method.

Molecular Mass

The recombinant human IL-7 protein predicts a molecular mass of 43.8 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state. 

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

IL-7 is an important cytokine for the growth, survival, and differentiation of T, B, and NK cells. The heterodimer formed by IL-7 and hepatocyte growth factor (HGF) is a precursor B cell growth stimulating factor. Studies on gene knockout in mice have shown that IL-7 plays a crucial role in the survival of lymphocytes. IL-7 stimulates pluripotent stem cells to differentiate into lymphoid progenitor cells.

References

1. Aliyari Z, Alemi F, Brazvan B, Tayefi Nasrabadi H, Nozad Charoudeh H. CD26+ Cord Blood Mononuclear Cells Significantly Produce B, T, and NK Cells. Iran J Immunol. 2015;12(1):16–26.

2. H.R. Kim, K.A. Hwang, S.H. Park, I. Kang.IL-7 and IL-15: biology and roles in T-Cell immunity in health and disease.Crit Rev Immunol, 28 (2008), pp. 325-339.

3.Su N, Shi SX, Zhu X, Borazanci A, Shi F, Gan Y. Interleukin-7 expression and its effect on natural killer cells in patients with multiple sclerosis. J Neuroimmunol (2014) 276:180–6.