Recombinant Human IL-21 Protein
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Business Type:Others
Country/Region:China
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8/10
T&L Biotechnology
We are professional supplier of Recombinant human IL-7 protein,Recombinant Human IL-15 Protein,Recombinant Human IL-21 Protein,Humanized Anti-Human CD3 mAb,Humanized Anti-Human CD16 mAb,Humanized Anti
Business Type:Others
Country/Region:China
Ddu Verified
HOT Rank
8/10
Catalog Number: GMP-TL-509
Product Name | |
Generic Name | Recombinant Human IL-21 Protein |
Synonym | CVID11, IL-21, Za11 |
Product Information | |
Protein sequence | A DNA sequence encoding the extracellular domain of human IL-21(Q9HBE4-1:Q29-S162)was expressed with the C-terminal fused Fc region of human IgG1. |
Expression Host | CHO cells |
QC Testing Purity | > 95 % as determined by SDS-PAGE |
Activity | Determined by inducing human natural killer lymphoma NK-92 cells to secrete interferon-γ. The expected ED50 is ≤ 50 ng/ml |
Endotoxin Level | < 0.01 EU per μg of the protein as determined by the LAL method. |
Molecular Mass | The recombinant human IL-21 predicts a molecular mass of 41.9 Kda. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
Stability & Storage | 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
Background | |
IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include: inducing the differentiation of T-cell-stimulated B-cells into plasma cells and memory B-cells; the stimulation of IgG production in conjunction with IL-4; and the induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. | |
References | |
1.Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34. 2.Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10. 3.Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 .Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96. |
Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).