T&L Biotechnology

Product Name

Generic Name

Anti-Human CD16 mAb

Product Information

Expression Host

CHO cells

QC Testing Purity

> 95 % as determined by SDS-PAGE

Purification

Protein A purified from cell culture supernatant

Endotoxin Level

< 0.01EU per μg of the protein as determined by the LAL method

Formulation

Supplied as a 0.22μm filtered solution in PBS, PH 7.4.

Stability & Storage

24 months at 2℃ to 8℃.

Avoid repeated freeze-thaw cycles.

Applications

Soluble method: The recommended concentration is 500 ng/mL.

Background

CD16, or FcγRⅢ, is a glycoprotein with a molecular weight of 50 000~70 000. It is a member of the Ig superfamily and is known to be involved in antibody-dependent cytotoxicity (ADCC) action. Its most significant feature is to trigger the lysis of target cells. As a low affinity receptor,CD16 can bind to the Fc ε association receptor I (FcεRI ) γ chain of CD3 ζ to participate in signal transduction. Cross-linked CD16 on NK cells directly leads to an increase of intracellular Ca2 + levels and a serial cascade of biochemical responses similar to the activation of T cell. According to the relevant literature, CD16 could mediate the direct killing of certain viral infected cells or tumor cells,which depends on the tight binding with antibodies. CD16 molecules are widely expressed in NK cells, neutrophilic polymorphonuclear leukocytes, mononuclear cells, and macrophages. CD16 monoclonal antibodies can activate NK cell and trigger antibody-dependent cell-mediated cytotoxic effects.

References 

1. B L Jin , J Q Guo , Y P Han , F Li , Q Zhao , W L Li , D M Zhang , H Wang , J P Zhang ,Optimization of the method to cultivate NK cells from abandoned white cells. Zhonghua Zhong Liu Za Zhi. 2016 Feb;38(2):105-12.

2. Maria Michela D'Aloia , Sara Caratelli , Camilla Palumbo , Simone Battella , Roberto Arriga , Davide Lauro , Gabriella Palmieri , Giuseppe Sconocchia , Maurizio Alimandi ,T lymphocytes engineered to express a CD16-chimeric antigen receptor redirect T-cell immune responses against immunoglobulin G-opsonized target cells. Cytotherapy . 2016 Feb;18(2):278-90.

3. Simone Battella , Maria Christina Cox , Angela Santoni , Gabriella Palmieri ,Natural killer (NK) cells

and anti-tumor therapeutic mAb: unexplored interactions. J Leukoc Biol. 2016 Jan;99(1):87-96.