T&L Biotechnology

Product Name

Generic Name

Recombinant Human BMP-2 Protein

Synonym

Bone Morphogenetic Protein-2, BMP-2A

Product Information

Protein sequence

A DNA sequence encoding the human BMP-2 (NP_001191.1) was expressed with a His-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC-5 mouse chondrogenic cells. The ED50 for this effect is ≤1 μg/mL.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The recombinant human BMP-2 contains 352 amino acids with a predicted molecular weight of 39.8 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state.

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

BMP-2 belongs to the transforming growth factor-βsuperfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine that can be associated with collagen combined with synthetic hydroxyapatite and other bone conduction vectors to induce the formation of bone and cartilage. It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.

References 

1. Developmental pathways of periodontal tissue regeneration: Developmental diversities of tooth morphogenesis doalso map capacity of periodontal tissue regeneration? Ripamonti U.J Periodontal Res. 2018 Sep 12. doi:10.1111/jre.12596.

2. Use of Recombinant Human Bone Morphogenetic Protein-2 Associated With Lyophilized Bovine Bone in

Reconstruction of Atrophic Maxilla.Zanettini LMS, Vieira F, Fritscher GG, Pagnoncelli RM, Heitz C.J Craniofac Surg.2018 Sep 10. doi: 10.1097/SCS.0000000000005049.

3. The Posterior Use of BMP-2 in Cervical Deformity Surgery Does Not Result in Increased Early Complications: AProspective Multicenter Study.Iyer S, Kim HJ, Bao H, Smith JS, Gupta M, Albert TJ, Protopsaltis TS, Mundis GM,Passias P, Neuman BJ, Klineberg EO, Lafage V, Ames CP.Global Spine J. 2018 Sep;8(6):622-628. doi:

10.1177/2192568218760538. Epub 2018 Mar 26..