T&L Biotechnology

Product Name

Generic Name

Recombinant Human IL-11Protein

Synonym

AGIF(Adipogenesis inhibitory factor),IL-11

Product Information

Construction

A DNA sequence encoding the human IL-11(NP_000632.1)was expressed with a His-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Determined by the dose-dependent stimulation of the proliferation of TF-1 cells.The expected ED 50 for this effect is ≤ 5 ng/ml.

Endotoxin

< 0.1EU per 1μg of the protein by the LAL method.

Molecular Mass

The recombinant human IL-11 protein consists of 184 amino acids and predicts a molecular mass of 20 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state. 

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

IL-11 is a multifunctional cytokine produced by fibroblasts, epithelial cells, osteoclast and other stromal cells. It is expressed in various tissues, including the thymus, lungs, bones, connective tissue, and central nervous system. IL-11 plays an important regulatory role in the hematopoietic process by stimulating the growth of bone marrow, red blood cells, and megakaryocyte progenitor cells. It also regulates bone metabolism, inhibits the production of pro-inflammatory cytokines, and prevents damage to the gastric mucosa.

References 

1. Biofloc technology (BFT): An alternative aquaculture system for prevention of Cyprinid herpesvirus 2 infection in gibel carp (Carassius auratus gibelio).Qiao G, Zhang M, Li Y, Xu C, Xu DH, Zhao Z, Zhang J, Li Q.Fish Shellfish Immunol. 2018 Sep 8. pii: S1050-4648(18)30551-5. doi: 10.1016/j.fsi.2018.09.015.

2. Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state.Chhana A, Pool B, Callon KE, Tay ML, Musson D, Naot D, McCarthy G, McGlashan S, Cornish J, Dalbeth N.Arthritis Res Ther. 2018 Sep 10;20(1):208. doi: 10.1186/s13075-018-1704-y.

3. Zac1 regulates IL-11 expression in osteoarthritis.Kuo CL, Liu ST, Chang YL, Wu CC, Huang SM.Oncotarget. 2018 Aug 21;9(65):32478-32495. doi: 10.18632/oncotarget.25980. eCollection 2018 Aug 21.