T&L Biotechnology

Recombinant Human MCP-1 (CCL2)

Catalog Number: GMP-TL630

Gene Name Synonym: GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,CMC-CF

Construction: A DNA sequence encoding the human MCP (NP_002973.1) was expressed with a polyhistidine tag at the C- terminus.

Source:Human

Expression Host: HEK293 cells

QC Testing Purity: <95 %, by SDS-PAGE and HPLC analyses

Endotoxin: <0.01 EU per μg of the protein by the LAL method.

Molecular Mass: The recombinant human MCP-1(CCL2) consists of 82 amino acids and predicts a

molecular mass of 9.5 KDa.

Formulation: Lyophilized from sterile PBS, pH 7.4. 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.

Stability & Storage:  Samples are stable for up to 24 months from date of receipt at 4 ℃. Samples are stable for 6-12 months at -20 ℃ after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage.

Avoid repeated freeze-thaw cycles.

Protein Description

The MCP proteins are members of the CC chemokine family and signals through CCR 2 and other CCR receptors except MCP-1. MCP proteins chemoattract and activate monocytes, T cells, basophils, NK cells and immature dendritic cells. The MCP family cross-reacts across species. Recombinant human MCP-1 is an 8.6 kDa protein containing 76 aminoa acid residues including the four highly conserved cysteine residues present in the CC chemokines.

References

1. Comparative Analysis of Human Adipose-Derived Mesenchymal Stem Cells from Orbital and Abdominal Fat. Nepali S, Park M, Lew H, Kim O. Stem Cells Int. 2018 Aug 19;2018:3932615. doi: 10.1155/2018/3932615. eCollection 2018.
2. Kallistatin Inhibits Atherosclerotic Inflammation by Regulating Macrophage Polarization. Li B, Sheng Z, Liu C, Qian L, Wu Y, Wu Y, Ma G, Yao Y. Hum Gene Ther. 2018 Sep 11. doi: 10.1089/hum.2018.084.
3. Anti-IFN-γ Antibody Promotes Osteoclastogenesis in Human Bone Marrow Monocyte-Derived Macrophages Co-Cultured with Tuberculosis-Activated Th1 Cells. Deng J, Sun D, Luo F, Zhang Q, Chen F, Xu J, Zhang Z. Cell Physiol Biochem. 2018 Sep 11;49(4):1512-1522. doi: 10.1159/000493455.
4. Differential regulation of cytokine and chemokine expression by MK2 and MK3 in airway smooth muscle cells. Ba M, Rawat S, Lao R, Grous M, Salmon M, Halayko AJ, Gerthoffer WT, Singer CA. Pulm Pharmacol Ther. 2018 Sep 8. pii: S1094-5539(18)30161-5. doi: 10.1016/j.pupt.2018.09.004.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).