T&L Biotechnology

Recombinant Human PDGF-AA

Catalog Number: GMP-TL743

Gene Name Synonym: Platelet-derived Growth Factor-AA, Glioma-derived Growth Factor (GDGF)

Osteosarcoma-derived Growth Factor (ODGF) .

Construction: A DNA sequence encoding the human PDGF-AA (NP_002598.4) was expressed with the C-terminal fused Fc region of human IgG1.

Source:Human

Expression Host: HEK293 Cells

QC Testing Purity: >95 % as determined by SDS-PAGE

Bio Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 2 ng/ml.

Endotoxin: <0.01 EU per μg of the protein as determined by the LAL method.

Molecular Mass: The recombinant human PDGF-AA consists of 358 amino acids and predicts a molecular mass of 41.2 KDa.

Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol

are added as protectants before lyophilization.

Stability & Storage: Samples are stable for up to 24 months from date of receipt at 4 ℃.

Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Protein Description

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB.

References

1. Selective estrogen receptor modulators enhance CNS remyelination independent of estrogen receptors KA Rankin, F Mei, K Kim, YA Shen, SR Mayoral, C Desponts, DS Lorrain, AJ Green, SE Baranzini, JR Chan, R Bove; J. Neurosci., 2019;0(0).

2. Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation; M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman; Cell Stem Cell, 2018;0(0).

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).