T&L Biotechnology

Product Name

Generic Name

Recombinant Human BMP-4 Protein

Synonym

Bone Morphogenetic Protein-4, BMP-2B, DVR4

Product Information

Protein sequence

A DNA sequence encoding the human BMP-4 (NP_001334841.1) was expressed with a Fc-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC-5 mouse chondrogenic cells. The ED50 for this effect is ≤50 ng/mL.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The recombinant human BMP-4 consists of 354 amino acids and predicts a molecular mass of 40.1 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state.

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL.

Avoid repeated freeze-thaw cycles.

Background

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. Members of this superfamily are widely distributed throughout the body, and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.

References 

1. Van den Wijngaard A, Weghuis DO, Boersma CJ, van Zoelen EJ, Geurts van Kessel A, Olijve W (Nov 1995). Fine mapping of the human bone morphogenetic protein-4 gene (BMP4) to chromosome 14q22-q23 by in situ hybridization. Genomics 27 (3): 559–60. doi:10.1006/geno.1995.1096. PMID 7558046.

2. Oida S, Iimura T, Maruoka Y, Takeda K, Sasaki S (Nov 1995). Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library. DNA Seq 5 (5): 273–5. doi:10.3109/10425179509030980. PMID 7579580.

3. Knöchel S, Dillinger K, Köster M, Knöchel W (November 2001). Structure and expression of Xenopus tropicalis BMP-2 and BMP-4 genes. Mech. Dev. 109 (1): 79–82.