T&L Biotechnology

Recombinant Human BMP-5

Catalog Number:GMP-TL647

Gene Name Synonyms: Bone Morphogenetic Protein-5

Construction: A DNA sequence encoding the human BMP-5 (NP_066551.1) was expressed with a polyhistidine tag at the C- terminus.

Species: Human

Expression Host: HEK293 cells

Testing Purity: ≥ 95 % as determined by SDS- PAGE & HPLC

Biological Activity: The ED50, as measured by its ability to induce alkaline phosphatase production by ATDC5 cells is expected as 1.0-2.0 ug/ml.

Endotoxin Level: ≤ 0.01 EU per μg of the protein by the LAL method.

Molecular Mass: The recombinant human BMP-5 consists of 376 amino acids and predicts a molecular mass of 42.6 KDa.

Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.

Stability & Storage:Stable for up to 24 months from date of receipt at 4 ℃. Stable for 6-12 months at -20℃ after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage and avoid repeated freeze-thaw cycles.

Protein Description

Transforming growth factor-β family members are key regulators of cell proliferation, differentiation, matrix synthesis and apoptosis. As their name implies, BMP family factors initiate, promote and regulate the development, growth and remodeling of bone and cartilage. In addition to this role, BMP family factors are also involved in prenatal and postnatal growth, remodeling and maintaining various other tissues and organs. BMP-5 is expressed in the nervous system, lungs and liver. It is a regulator of the growth of sympathetic neuron dendritic cells. BMP-5 is a precursor protein of 454 amino acids, which is cleaved to release a biologically active C-terminal mature protein.

References

1. Screening of key molecules of HAb18G/CD147 signal transduction pathway in human hepatoma cells. Zhou H, Yao Y, Liang Y, Wang Z, Zhou X, Guo W, Yang X, Jiang J, Sun J, Chen Z. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Sep;33(9):1196-1200. Chinese.
2. High serum levels of BMP-2 correlate with BMP-4 and BMP-5 levels and induce reduced neuronal phenotype in patients with relapsing-remitting multiple sclerosis. Penn M, Mausner-Fainberg K, Golan M, Karni A. J Neuroimmunol. 2017 Sep 15;310:120-128. doi:10.1016/j.jneuroim.2017.07.008. Epub 2017 Jul 15.
3. The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway. Tian XP, Jin XH, Li M, Huang WJ, Xie D, Zhang JX. Mol Cancer. 2017 Apr 4;16(1):74. doi: 10.1186/s12943-017-0637-4.

4. Correlation between expression of long non-coding RNA ZXF1 and prognosis of lung adenocarcinoma and its potential molecular mechanism. Pan GF, Zhou XF, Zhao JP.Zhonghua Zhong Liu Za Zhi. 2017 Feb 23;39(2):102-108. doi: 10.3760/cma.j.issn.0253-3766.2017.02.006.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).