T&L Biotechnology

Product Name

Generic Name

Recombinant Human IL-1α Protein

Synonym

L1A, IL-1α, IL1F1, IL1α

Product Information

Protein sequence

A DNA sequence encoding the human IL-1α（P01583-1：S113-A271）was expressed with a His-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Determined by the level of  interferon-γ secreted from induce human natural destruction lymphoma NK-92 cells.The expected ED 50 for this effect is ≤ 100 ng/ml.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The recombinant human IL-1αprotein predicts a molecular mass of 18.9 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state. 

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

Interleukin-1 (IL-1) is a peptide with a molecular weight of 17 kD and the presence of IL-1αand IL-1βtwo subtypes. IL-1αcontains 159 amino acids; IL-1β contains 153 amino acids; Both are encoded by different genes. Although its amino acid sequence has only 26% homology, IL-1αand IL-1βbind to the same cell surface receptors with the same affinity and exert the same biological effects. IL-1 receptors (IL-1R) are present on almost all nucleated cell surfaces, with varying numbers of IL-1R per cell, ranging from tens (such as t cells) to thousands (such as fibroblasts). There are two main types of IL-1R: one is IL-1r1, which has a longer peptide chain extending into the cytoplasm and plays a role in transmitting activation signals; The other is IL-1R2, which The peptide segment of the intracellular part is short, which can not effectively transmit signals. Instead, it releases the peptide chain of the extracellular part into extracellular fluid, and combines with IL-1 in a free form to play a feedback inhibitory role. GM-CSF, G-CSF, and IL-1 can all increase the expression level of IL-1R in cells, while TGF and corticosteroids can reduce the expression of IL-1R.

References 

1. Nicklin MJ, Weith A, Duff GW (1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics 19 (2): 382–4. doi:10.1006/geno.1994.1076.

2. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, Gillis S, Henney CS, Kronheim SR, Grabstein K (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315 (6021): 641–7. doi:10.1038/315641a0.

3. Bankers-Fulbright JL, Kalli KR, McKean DJ (1996) Interleukin-1 signal transduction. Life Sciences 59 (2): 61–83. doi:10.1016/0024-3205(96)00135-X.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).