T&L Biotechnology

Product Name

Generic Name

Recombinant Human IL-3 Protein

Synonym

MCGF(Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

Product Information

Protein sequence

A DNA sequence encoding the human IL-3 (GenBank: AAA59146.1) was expressed with a His-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is ≤ 5 ng/mL.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The recombinant human IL-3 protein consists of 139 amino acids and predicts a molecular mass of 15.9 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state.

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of megakaryocytes, granulocyte-macrophages, erythrocytes, eosinophils, basophils, and progenitors of the mast cell line. IL-3 is produced by T cells, mast cells, and eosinophils and enhances platelet production, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunity epidemic regulation. Much of the activity of IL-3 depends on costimulation with other cytokines. IL-3 is a species-specific, mutable glycosylated cytokine.

References 

1. Loss of solute carrier family 7 member 2 exacerbates inflammation-associated colon tumorigenesis. Coburn LA, Singh K, Asim M, Barry DP, Allaman MM, Al-Greene NT, Hardbower DM, Polosukhina D, Williams CS, Delgado AG, Piazuelo MB, Washington MK, Gobert AP, Wilson KT. Oncogene. 2018 Sep 10. doi: 10.1038/s41388-018-0492-9.

2. Irreversible effects of trichloroethylene on the gut microbial community and gut-associated immune responses in auto immune-prone mice. Khare S, Gokulan K, Williams K, Bai S, Gilbert KM, Blossom SJ.J Appl Toxicol. 2018 Sep 5. doi 10.1002/jat.3708.

3. Comparative utility of NRG and NRGS mice for the study of normal hematopoiesis, leukemogenesis, and therapeutic response.Barve A, Casson L, Krem M, Wunderlich M, Mulloy JC, Beverly LJ. Exp Hematol. 2018 Aug 17. pii: S0301-4 72X(18)30751-3. doi: 10.1016/j.exphem.2018.08.004.