T&L Biotechnology

Product Name

Generic Name

Recombinant Human OX40 Ligand Protein

Synonym

OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1

Product Information

Protein sequence

A DNA sequence encoding the human OX40 Ligand (NCBI Reference Sequence: NP_003317.1) was expressed with a Fc-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Measured by its ability to induce IL-8 secretion by PBMC. The ED50 for this effect is ≤ 100 ng/mL.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The recombinant human OX40 Ligand protein consists of 364 amino acids and predicts a molecular mass of 42.4 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state.

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

References 

1. OX40 Ligand Contributes to Human Lupus Pathogenesis by Promoting T follicular Helper Response Clément Jacquemin, Nathalie Schmitt, Cécile Contin-Bordes, Yang Liu, Priya Narayanan, Julien Seneschal, Typhanie Maurouard, David Dougall, Emily Spence Davizon, Hélène Dumortier, Isabelle Douchet, Loïc Raffray, Christophe Richez, Estibaliz Lazaro, Pierre Duffau, Marie-Elise Truchetet, Liliane Khoryati, Patrick Mercié, Lionel Couzi, Pierre Merville, Thierry Schaeverbeke, Jean-François Viallard, Jean-Luc Pellegrin, Jean-François Moreau, Sylviane Muller, Sandy Zurawski, Robert L. Coffman, Virginia Pascual, Hideki Ueno, Patrick Blanco Immunity. Author manuscript; available in PMC 2016 Jun 16. Published in final edited form as: Immunity. 2015 Jun 16; 42(6):1159–1170. Published online 2015 Jun 9. doi: 10.1016/j.immuni.2015.05.012.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).