T&L Biotechnology

Product Name

Generic Name

Recombinant Human SCF Protein

Synonym

C-kitligand, DCUA, FPH2, FPHH, Kitl, KL-1, MGF, SCF, SF, SHEP7

Product Information

Protein sequence

A DNA sequence encoding the human SCF (NP_000890.1) was expressed with a His-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Measured in a cell proliferation assay using TF‑1 cells. The ED50 for this effect is ≤ 15 ng/mL.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The recombinant human SCF protein consists of 170 amino acids and predicts a molecular mass of 19.3 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state.

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

Stem Cell Factor, also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, is a dimeric molecule that exerts its biological functions by binding to and activating the receptor tyrosine kinase c-Kit. Activation of c-Kit leads to its autophosphorylation and initiation of signal transduction. Signaling proteins are recruited to activated c-Kit by certain interaction domains that specifically bind to phosphorylated tyrosine residues in the intracellular region of c-Kit. Binding of SCF to C-kit induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain. Tyrosine phosphorylation initiates multiple signaling pathways including RAS, PI3 kinase, Src, and JAK/STAT. However, SCF is a versatile factor in the differentiation of many specific cell types like spermatogonial stem cells and megakaryocyte progenitors. Apart from differentiation, SCF also can maintain stemness in cells. For clinical application, SCF is used in combination with other cytokines to generate myeloid-derived suppressor cells from human umbilical cord blood. SCF is also used to generate T cells for cell-based therapies, drug screening and disease modeling. In regenerative studies, SCF is applied in wound healing hydrogel as a means of increasing its adhesion strength and tissue regeneration.

References 

1. Geissler EN, Liao M, Brook JD, Martin FH, Zsebo KM, Housman DE, Galli SJ (March 1991). "Stem cell factor (SCF), a novel hematopoietic growth factor and ligand for c-kit tyrosine kinase receptor, maps on human chromosome 12 between 12q14.3 and 12qter". Somat. Cell Mol. Genet. 17 (2): 207–14. doi:10.1007/BF01232978. PMID 1707188.

2. Flanagan JG, Chan DC, Leder P (March 1991). "Transmembrane form of the kit ligand growth factor is determined by alternative splicing and is missing in the Sld mutant". Cell 64 (5): 1025–35. doi:10.1016/0092-8674(91)90326- t. PMID 1705866.

3. Anderson DM, Williams DE, Tushinski R, Gimpel S, Eisenman J, Cannizzaro LA, Aronson M, Croce CM, Huebner K, Cosman D (August 1991). "Alternate splicing of mRNAs encoding human mast cell growth factor and localization of the gene to chromosome 12q22-q24". Cell Growth Differ. 2 (8): 373–8. PMID 1724381.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).