T&L Biotechnology

Product Name

Generic Name

Recombinant Human Vitronectin Protein

Synonym

V75, Vitronectin, VN, VTN, S-protein, Serum-spreading factor

Product Information

Protein sequence

A DNA sequence encoding the human Vitronectin（NP_000629.3）was expressed with a polyhistidine tag at the C-terminus.

Expression Host

HEK293 Cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Vitronectin promotes attachment of hESCs and iPSCs with 5 μg/mL under the condition of serum-free feeding .

Endotoxin Level

< 0.1EU per μg of the protein as determined by the LAL method.

Molecular Mass

The Recombinant Human Vitronectin consists of 465 amino acids and predicts a molecular mass of 53 KDa.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6% mannitol are added as protectants before lyophilization.

Stability & Storage

Samples are stable for up to 24 months from date of receipt at 4 ℃.

Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Usage method

1. Reconstitute each 20mg of vitronectin with 1mL of sterilized water, mix well and stand for 25~30 minutes until dissolving. Then mix well, aliquot and freeze.

2. Dilute vitronectin with sterile phosphate buffer (PBS) to obtain the desired concentration and dissolve vitronectin by gentle pipetting.

Note: Do not vortex vigorously; Different concentrations of dilutions need to be tested to determine the optimal concentration for each culture system. Recommended coating concentration range is 5~50 μg/mL.

3. Taking 6-well-plate as an example, add 1mL of diluted vitronectin solution to each well, and leave the plate at room temperature for 1~2 hours.

4. After the plate is completely coated, aspirate PBS and discard it, and gently wash the plate with sterile PBS.

Background

Recombinant human vitronectin protein efficiently binds to extracellular matrices and is primarily used to promote cell adhesion by coating plates or flasks. In the matrix, vitronectin can support cell adhesion by binding to various integrins and other proteoglycans. In addition, vitronectin can function as a chemically defined matrix component in human embryonic stem cell regeneration media.

References 

1. Evaluation of potential effects of Plastin 3 overexpression and low-dose SMN-antisense oligonucleotides on putative biomarkers in spinal muscular atrophy mice.Strathmann EA, Peters M, Hosseinibarkooie S, Rigo FW, Bennett CF, Zaworski PG, Chen KS, Nothnagel M, Wirth B.PLoS One. 2018 Sep 6;13(9):e0203398. doi: 10.1371/journal.pone.0203398. eCollection 2018.

2. Detection of Complement Activators in Immune Attack Eyes After iPS-Derived Retinal Pigment Epithelial Cell Transplantation.Sugita S, Makabe K, Fujii S, Takahashi M.Invest Ophthalmol Vis Sci. 2018 Aug 1;59(10):4198-4209. doi: 10.1167/iovs.18-24769.

3. Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.Chen YH, Pruett-Miller SM.Stem Cell Res. 2018 Aug;31:186-192. doi: 10.1016/j.scr.2018.08.003. Epub 2018 Aug .

4. Bacterial Outer Membrane Vesicles Induce Vitronectin Release Into the Bronchoalveolar Space Conferring Protection From Complement-Mediated Killing.Paulsson M, Che KF, Ahl J, Tham J, Sandblad L, Smith ME, Qvarfordt I, Su YC, Lindén A, Riesbeck K.Front Microbiol. 2018 Jul 13;9:1559. doi: 10.3389/fmicb.2018.01559. eCollection 2018.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).