T&L Biotechnology

Product Name

Generic Name

Recombinant Human IL-6 Protein

Synonym

IL6,Interleukin-6,BSF2,HSF,IFNB2

Product Information

Protein sequence

A DNA sequence encoding the human IL-6（NP_000591.1：V30-M212）was expressed with a His-tag at the C-terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Determined by the dose-dependent stimulation of the proliferation of TF-1 cells. The expected ED 50 for this effect is ≤1ng/mL.

Endotoxin Level

< 0.1EU per μg of the protein as determined by the LAL method.

Molecular Mass

The Recombinant Human IL-6 Protein predicts a molecular mass of 21.6 kD.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state. 

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

Interleukin 6 (IL-6) produced by fibroblasts, monocytes, macrophages, T lymphocytes, B lymphocytes, epithelial cells,  keratinocytes and various tumor cells. IL-1, TNF-α, PDGF, viral infection, double stranded RNA, and cAMP can all induce normal cells produce IL-6. IL-6 can stimulate the proliferation, differentiation of immune responses and improve immune cell functions. IL-6R is widely expressed on the surface of activated B cells, quiescent T cells, NK cells, myeloma cells, hepatocytes, and myeloid leukemia cells. The main role of IL-6 is to promote the proliferation and differentiation of B cells and the secretion of antibodies. It also has extensive effects on liver cells, T cells, neural tissue, and hematopoietic system. IL-6 also has anti-tumor effects and can directly or indirectly enhance the tumoricidal activity of NK cells and CTL.

References 

1. Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB, Ruddle FH (1988)Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15-p21. Genomics 2 (3): 203–8. doi:10.1016/0888-7543(88)90003-1. PMID 3294161.

2. Van der Poll T, Keogh CV, Guirao X, Buurman WA, Kopf M, Lowry SF (1997)Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. The Journal of Infectious Diseases 176 (2): 439–44. doi:10.1086/514062. PMID 9237710.

3. Banks WA, Kastin AJ, Gutierrez EG (1994)Penetration of interleukin-6 across the murine blood-brain barrier. Neuroscience Letters 179 (1-2): 53–6. doi:10.1016/0304-3940(94)90933-4. PMID 7845624.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).