T&L Biotechnology

Product Name

Generic Name

Recombinant Human IL-12 Protein

Synonym

IL12, p70, Interleukin-12

Product Information

Protein sequence

Recombinant human IL-12 is heterodimeric and composed of P29459-1 (IL12A) and P29460-1 (IL12B).

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Activity

Determined by the dose-dependent secretion of interferon γ by human naturally killer lymphoma NK-92 cells.The expected ED ₅₀ for this effect is ≤ 2 ng/ml.

Endotoxin Level

< 0.1EU per 1 μg of the protein by the LAL method.

Molecular Mass

The Recombinant Human IL-12 Protein predicts a molecular mass of 65 kDa. 

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state. 

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

IL-12 is primarily produced by monocytes, macrophages, B cells and DCs. IL-12 is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40).IL-12 receptors are located mainly on T cells and NK cells, and IL-12 can stimulate Th1 and NK cell growth, while inhibiting Th2 cell responses. IL-12 augments cytotoxicity of T lymphocytes and NK cells through the enhancement of IFN-γ and TNF-α secretion, and inhibition of IL-4 production.

References 

1. Frazão JB, Colombo M, Simillion C, Bilican A, Keller I, Wüthrich D, Zhu Z, Okoniewski MJ, Bruggmann R, Condino-Neto A, Newburger（2019）Gene expression in chronic granulomatous disease and interferon-γ receptor-deficient cells treated in vitro with interferon-γ. J Cell Biochem.120(3):4321-4332.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).