T&L Biotechnology

Product Name

Generic Name

Recombinant Human TNF α Protein

Synonym

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNF-α

Product Information

Protein sequence

A DNA sequence encoding the human TNF-α (GenBank: ADV31546.2 V77-L233) was expressed with a polyhistidine tag at the C- terminus.

Expression Host

HEK293 cells

QC Testing Purity

> 90% as determined by SDS-PAGE

Activity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of  actinomycin D. The ED₅₀ for this effect is ≤5 ng/mL.

Endotoxin Level

< 0.1EU per μg of the protein as determined by the LAL method.

Molecular Mass

The Recombinant protein predicts a molecular mass of 18.2 KDa.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.

Stability & Storage

24 months at 2℃ to 8℃ in lyophilized state. 

6 months at -20℃ under sterile conditions after reconstitution.

12 months at -80℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.

Avoid repeated freeze-thaw cycles.

Background

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. TNF-α is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is involved in fighting against tumorigenesis. It is regarded as a potential protein of cancer treatment.

References 

1. Hector J, et al. (2007) TNF-α alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.

2. Berthold-Losleben M, et al. (2008) The TNF-α System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.

3. Zelová H, Hošek J (2013) TNF-alpha signalling and inflammation: interactions between old acquaintances. Inflamm Res 62: 641–651

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).