T&L Biotechnology

Product Name

Generic Name

Anti-Human CD52 mAb

Product Information

Expression Host

HEK293 cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Purification

Protein A purified from cell culture supernatant

Endotoxin Level

< 0.1EU per μg of the protein as determined by the LAL method

Formulation

Supplied as a 0.22μm filtered solution in PBS, PH 7.4.

Stability & Storage

24 months at 2℃ to 8℃.

Avoid repeated freeze-thaw cycles.

Background

CD52 is a widely distributed antigen belonging to a GPI anchored glycoprotein with only twelve amino acid residues (GQNDTSQTSSPS). CD52 can be found on the surface of lymphocytes, monocytes,eosophils and dendritic cells in hematopoietic system. with a density of up to 5×10⁵ molecules on lymphocytes, CD52 antigens express on many lymphoid cell malignancies and certain acute myeloid leukemia cells. When CD52 molecules are crosslinked with mAb, it triggers a series of signaling within the target cells.The signaling caused by the activation of CD52 molecules is similar to that caused by anti-CD3 antibodies. When CD52 molecules are triggered, PMA (Phorbol Myristate acetate, acetylmyridrobol ester) can promote the proliferation and secretion of cytokines in normal human CD4 + and CD8 + T lymphocytes in vitro.

References 

1. Quigley MM, Bethel KJ, Sharpe RW.CD52 expression in hairy cell leukemia[ J]. Am J Hematol, 2003 , 74(4):227-230.

2. Rowan W, Tite J, Topley P .Cross-linking of the CAMPATH-1 antigen (CD52)mediates growth inhibition in human B- and T- lymphoma cell lines, and subsequent emergence of CD52-deficient cells[ J] .Immunology , 1998 ,95:427-436. [J]. Immunology, 1998,95:427-436.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).