T&L Biotechnology

Product Name

Generic Name

Anti-Human HER-2 mAb

Product Information

Expression Host

CHO cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Purification

Protein A purified from cell culture supernatant

Endotoxin Level

<0.1EU/μg

Formulation

Supplied as a 0.22μm filtered solution in PBS, PH 7.4.

Stability & Storage

24 months at 2℃ to 8℃.

Avoid repeated freeze-thaw cycles.

Background

HER 2, also known as ErbB 2, is one of the members of the human epidermal growth factor receptor family and has important signal transduction roles. Her-2 / neu protein-mediated signal transduction pathway mainly includes Ras / Raf / separin-activated protein kinase (MAPK) pathway, phosphatidylinositol 3 hydroxykinase (P13K) / Akt pathway, signal transduction and transcriptional activation (STAT) pathway and PLC pathway etc. Her-2 / neu protein is usually expressed only in fetuses at low levels within a very few tissues after adulthood. Her-2 / Neu expression was found in various human tumors, such as breast, ovarian, lung adenocarcinoma, primary renal cell carcinoma, and endometrial cancer. In gastric cancer cells, Her-2 / neu protein was mainly localized on cell membrane and was minimally expressed in the cytoplasm.

References 

1. Mitri Z, Constantine T, O'Regan R (2012). "The HER2 Receptor in Breast Cancer: Pathophysiology, Clinical Use, and New Advances in Therapy". Chemotherapy Research and Practice 2012: 743193. doi:10.1155/2012/743193. PMC 3539433. PMID 23320171.

2. Coussens L, Yang-Feng TL, Liao YC, Chen E, Gray A, McGrath J, Seeburg PH, Libermann TA, Schlessinger J, Francke U (Dec 1985)."Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene". Science 230 (4730): 1132–9. doi:10.1126/science.2999974. PMID 2999974.

3. Olayioye MA (2001). "Update on HER-2 as a target for cancer therapy: intracellular signaling pathways of ErbB2/HER-2 and family members". Breast Cancer Research 3 (6): 385–9. doi:10.1186/bcr327. PMC 138705. PMID 11737890.