T&L Biotechnology

Product Name

Generic Name

Anti-Human PD-1 mAb

Product Information

Expression Host

CHO cells

QC Testing Purity

> 90 % as determined by SDS-PAGE

Purification

Protein A purified from cell culture supernatant

Endotoxin Level

< 0.1EU per μg of the protein as determined by the LAL method

Formulation

Supplied as a 0.22μm filtered solution in PBS, PH 7.4.

Stability & Storage

24 months at 2℃ to 8℃.

Avoid repeated freeze-thaw cycles.

Background

PD-1 is mainly expressed in activated T or B cells and inhibit cell activation, which is a normal autostable mechanism of immune system. Excessive T / B cell activation can cause autoimmune diseases, so PD-1 is necessary in our body. But tumor microenvironment will induce high expression of PD-1 in infiltrating T cells. Tumor cells also express PD-L1 and PD-L2 and inhibit T cell which fail to kill tumor cells. PD-1 antibody can block this pathway, restore the function of T cells and make T cells continue to kill tumor cells. Therefore, anti-human PD-1 monoclonal antibody are applied to exert a "closed" effect on the expanded T cells in vitro, improving the tumor killing effect of immune cells.

References 

1. Shinohara T, Taniwaki M, Ishida Y, Kawaichi M, Honjo T (Oct 1994). "Structure and chromosomal localization of the human PD-1gene (PDCD1)". Genomics 23 (3): 704–6. doi:10.1006/geno.1994.1562. PMID 7851902.

2. Fife BT, Pauken KE (Jan 2011). "The role of the PD-1 pathway in autoimmunity and peripheral tolerance". Annals of the New York Academy of Sciences 1217: 45–59. doi:10.1111/j.1749- 6632.2010.05919.x. PMID 21276005.

3. Loftus, Peter (16 Nov 2014). "New Bristol-Myers Drug Helped Skin-Cancer Patients in Trial Live Longer". Retrieved 24 Nov 2014.Francisco LM, Sage PT, Sharpe AH (Jul 2010). "The PD-1 pathway in tolerance and autoimmunity". Immunological Reviews 236: 219–42. doi:10.1111/j.1600-065X.2010.00923.x. PMC 2919275. PMID 20636820.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).